| 质粒名称: | pCAG-HA-Nrxn1beta AS4(+) |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 59410 |
| 存储实验室: | Peter Scheiffele |
|
载体骨架基本信息 |
|
| 载体名称: | pCAG |
| 空载体大小: | 4867 bp |
| 完整质粒大小: | 6312 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Nrxn1beta AS4(+) / Neurexin 1beta AS4(+) / Neurexin 1beta Splice4 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 1443 bp |
| 启动子: | CAG |
| 标签/融合蛋白: | HA (N terminal on insert) |
| 克隆方法: | 未知 |
| 5’测序引物 | CAG-F (5'-GCAACGTGCTGGTTATTGTG-3') |
| 3’测序引物 | Bglob-pA-R (5'-TTTTGGCAGAGGGAAAAAGA-3'); pCAG_rev_seq (5'-CAGCCACCACCTTCTGATAG-3') |
|
文献引用方法 |
|
| 材料和方法部分: | pCAG-HA-Nrxn1beta AS4(+) was a gift from Peter Scheiffele (Addgene plasmid # 59410 ; http://n2t.net/addgene:59410 ; RRID:Addgene_59410) |
| 参考文献部分: | Alternative splicing controls selective trans-synaptic interactions of the neuroligin-neurexin complex. Chih B, Gollan L, Scheiffele P. Neuron. 2006 Jul 20. 51(2):171-8. 10.1016/j.neuron.2006.06.005 PubMed 16846852 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pCAG-HA-Nrxn1beta AS4(+) 6312 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(+)
[containing the insert at alternatively spliced segment 4 (AS4+)] .
ACCESSION .
VERSION .
KEYWORDS pCAG-HA-Nrxn1beta AS4(+)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6312)
AUTHORS Chih B, Gollan L, Scheiffele P
TITLE Alternative splicing controls selective trans-synaptic interactions
of the neuroligin-neurexin complex.
JOURNAL Neuron. 2006 Jul 20. 51(2):171-8.
PUBMED 16846852
REFERENCE 2 (bases 1 to 6312)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6312
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 368..645
/label=chicken beta-actin promoter
intron 647..1664
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1588..1610
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1672..1691
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
CDS 1909..1935
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
primer_bind complement(3316..3335)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 3381..3436
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(3435..3454)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(3797..3813)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3797..3813)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3810..3832)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3821..3837
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3845..3875)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3890..3911
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3970..4165
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 4016..4151
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4078..4097
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 4171..4305
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4208..4227)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4262..4281
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(4373..4390)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4544..5132)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4624..4643)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5303..6163)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5926..5945
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6164..6268)
/gene="bla"
/label=AmpR promoter
enhancer 6299..366
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
361 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
421 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
481 gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
541 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
601 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac
661 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
721 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
781 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
841 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
901 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
961 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
1021 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
1081 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
1141 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
1201 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
1261 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
1321 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
1381 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
1441 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
1501 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
1561 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
1621 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
1681 tggttattgt gctgtctcat cattttggca aagaattcgc ggccgctagc gctaccggac
1741 tcagatctcg agctcaagct tgggaccatg gcacttccca gatgcatgtg gccaaattat
1801 gtttggagag ctatgatggc atgtgtggtc cacaggggat ccggtgcccc attgactctc
1861 tgcttgttgg gatgtttgct acagactttt cacgtactct ctcaaaagta tccatacgac
1921 gttccggact acgcaggtac cgcatccagt ttgggagcgc accacatcca ccatttccat
1981 ggcagcagca agcatcattc agtgcctatt gcaatctaca ggtcaccagc atccttgcga
2041 ggcggacacg ctgggacaac atatatcttt agcaaaggtg gtggacagat tacatataag
2101 tggcctccca atgaccgccc cagtacacga gcagacaggc tggccatcgg atttagcact
2161 gtccagaagg aagccgtgtt ggtgcgtgtg gacagttcct caggcctggg tgactacctg
2221 gagctgcaca tacaccaagg aaaaattgga gttaagttta atgttgggac agatgacatc
2281 gccatcgaag agtctaatgc aatcattaat gatgggaaat accatgtagt acgtttcaca
2341 aggagtggtg gcaatgccac gttacaggtg gacagctggc cagctatcga acgctaccct
2401 gcaggaaaca atgataacga gcgcctggcg attgctagac agcgaattcc atatcgactt
2461 ggtcgagtag ttgatgaatg gctactcgac aaagggcgtc agctcacaat cttcaatagc
2521 caagcaacca taataattgg cgggaaagag cagggccagc ccttccaggg tcagctctct
2581 ggtctttact acaatggctt gaaagttctg aatatggcag cagagaacga tgccaacatc
2641 gccatagtgg gaaatgtgag gctggtcggt gaagtgcctt cctctatgac aactgagtcg
2701 acagccactg ccatgcagtc tgagatgtcc acctcaatca tggagaccac cacaaccctg
2761 gctaccagca cagctagaag aggcaagccc cccacaaagg aacccatcag ccagaccaca
2821 gatgacatcc ttgtggcctc ggcagagtgt cccagtgacg atgaggacat tgacccctgt
2881 gagccgagct caggtgggtt agccaacccc accagagtag gtggccgtga accataccca
2941 ggctctgcag aggtgattcg ggagtccagc agtaccactg gcatggtggt ggggattttc
3001 gcggcagccg ctctgtgcat cctcatcctc ctctatgcca tgtacaagta ctggaaccgg
3061 gatgaagggt cgtaccacgt ggatgagagt cgaaactaca tcagtaactc agcacagtcc
3121 aatggggctg tggtcaagga gaagcaaccc agcagtgcta aaagcgccaa caaaaacaag
3181 aagaacaagg ataaggagta ttatgtctga ctcgaggtac ccccggggag ctcaattcac
3241 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
3301 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
3361 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
3421 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
3481 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
3541 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
3601 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
3661 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
3721 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa
3781 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
3841 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
3901 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
3961 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
4021 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
4081 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
4141 ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac
4201 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
4261 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa
4321 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
4381 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
4441 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
4501 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
4561 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
4621 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
4681 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
4741 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
4801 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
4861 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
4921 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
4981 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
5041 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
5101 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
5161 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
5221 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
5281 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
5341 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
5401 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
5461 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
5521 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
5581 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
5641 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
5701 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
5761 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
5821 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
5881 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
5941 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
6001 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
6061 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
6121 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
6181 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
6241 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
6301 cattgattat tg
//
